Class 12 · Biology · CBSE Board · 2016–2025

Biotechnology – Principles and Processes — Class 12 Biology PYQs

81 questions from this chapter, asked in 9 Class 12 exams between 2016–2025 — every question paper set included, duplicates removed.

81questions

9Class 12 exams

2016–2025years covered

1 / 2 / 3 / 4 / 5mark values asked

Questions asked per year

202517 Qs 202415 Qs 202312 Qs 20228 Qs 20208 Qs 20198 Qs 201811 Qs 20175 Qs 20162 Qs

Practice questions first 10 of 81 — free

Q1MCQ20251 mark

Answer Logic

Identify the sticky ends in the DNA segment given below :
5' - GAATTCG - 3'
3' - CTTAAGC - 5'
[Max Marks: 1 | Set: 57/2/3]

(A)5' - G and 3' - C

(B)5' - AATTC and 3' - TTAAG

(C)5' - GAATTC and 3' - CTTAAG

(D)5' - EcoRI and 3' - EcoRI

Q2MCQ20251 mark

Answer Logic

A single stranded DNA or RNA tagged with a radioactive molecule is called :
[Max Marks: 1 | Sets: 57/2/3]

(A)Vector

(B)Plasmid

(C)Probe

(D)Clone

Q3MCQ20251 mark

Answer Logic

Which one of the following palindromic base sequences is cut by restriction endonuclease Hind II ?
[Max Marks: 1 | Sets: 57/1/2]

(A)5' - GGATCC - 3'
3' - CCTAGG - 5'

(B)5' - GAATTC - 3'
3' - CTTAAG - 5'

(C)5' - GTCGAC - 3'
3' - CAGCTG - 5'

(D)5' - GTPyPuAC - 3'
3' - CAPuPyTG - 5'

Q4MCQ20251 mark

Answer Logic

In pBR322, the gene that codes for the proteins involved in ampicillin resistance is :
[Max Marks: 1 | Sets: 57/1/3]

(A)ampR

(B)tetR

(C)rop

(D)ori

Q5MCQ20251 mark

Answer Logic

Which one of the following restriction enzymes produces sticky ends ?
[Max Marks: 1 | Sets: 57/2/1]

(A)SmaI

(B)EcoRV

(C)PvuII

(D)EcoRI

Q6MCQ20251 mark

Answer Logic

In genetic engineering, which enzyme is used to cut DNA at specific locations ?
[Max Marks: 1 | Sets: 57/2/2]

(A)DNA ligase

(B)DNA polymerase

(C)Restriction endonuclease

(D)Taq polymerase

Q7MCQ20251 mark

Answer Logic

Which one of the following palindromic sequences is recognised by EcoRI ?
[Max Marks: 1 | Sets: 57/1/1]

(A)5' - GAATTC - 3'

(B)5' - GGCC - 3'

(C)5' - AAGCTT - 3'

(D)5' - CCCGGG - 3'

Q8MCQ20251 mark

Answer Logic

Which of the following is not a characteristic of a cloning vector ?
[Max Marks: 1 | Sets: 57/2/1]

(A)Origin of replication

(B)Restriction sites

(C)Antibiotic resistance gene

(D)Gene of interest

Q9MCQ20251 mark

Answer Logic

What does 'ori' site in pBR322 do ?
[Max Marks: 1 | Sets: 57/1/2]

(A)It controls the copy number of the linked DNA

(B)It produces restriction endonuclease

(C)It produces DNA polymerase

(D)It digests foreign DNA

Q10MCQ20251 mark

Answer Logic

Which one of the following is not a desirable feature of a cloning vector ?
[Max Marks: 1 | Sets: 57/2/2]

(A)Origin of replication

(B)Selectable marker

(C)Multiple cloning sites

(D)Large size

Why practise Biotechnology – Principles and Processes PYQs?

Biotechnology – Principles and Processes has appeared in 9 Class 12 Biology exams we track between 2016–2025, with questions worth 1, 2, 3, 4, 5 marks. CBSE Board examiners consistently reuse concepts and question patterns from this topic — practising its previous year questions is the most reliable way to know exactly what to expect in your exam.

Biotechnology – Principles and Processes — Class 12 Biology PYQs

Questions asked per year

Practice questions first 10 of 81 — free

Why practise Biotechnology – Principles and Processes PYQs?

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